• Product nameAnti-Ndufs1 antibody
    See all Ndufs1 primary antibodies
  • Description
    Mouse polyclonal to Ndufs1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish
  • Immunogen

    Fusion protein:


    , corresponding to amino acids 104/203 of Mouse Ndufs1

  • General notesProduced from outbred CD1 mice

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.



Our Abpromise guarantee covers the use of ab22094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 79 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 20966424


  • FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
  • Involvement in diseaseDefects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similaritiesBelongs to the complex I 75 kDa subunit family.
    Contains 1 2Fe-2S ferredoxin-type domain.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI-75kD antibody
    • Complex I 75Kd antibody
    • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
    • Complex I-75kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
    • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
    • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
    • NDUFS1 antibody
    • NDUS1_HUMAN antibody
    • PRO1304 antibody
    see all

Anti-Ndufs1 antibody images

  • All lanes : Anti-Ndufs1 antibody (ab22094) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 79 kDa

References for Anti-Ndufs1 antibody (ab22094)

This product has been referenced in:
  • Suryawanshi AR  et al. Differential Proteomics Leads to Identification of Domain Specific Epididymal Sperm Proteins. J Androl : (2010). WB, ICC/IF ; Human, Rat . Read more (PubMed: 20966424) »

See 1 Publication for this product

Product Wall

Grazie per la sua richiesta.

Non abbiamo l'anticorpo secondarioesattonel nostro catalogo che era utilizzato nel Western blot sulla scheda tecnica. Purtroppo posso confermare che il ab22094 è un anticorpo policlonale del topo che sar&ag...

Read More