Overview

  • Product nameAnti-Ndufs1 antibody
    See all Ndufs1 primary antibodies
  • Description
    Mouse polyclonal to Ndufs1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Human, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus Monkey, Gorilla, Chlamydomonas reinhardtii, Orangutan
  • Immunogen

    Synthetic peptide:

    MFGSDRSRFLEGKRAVEDKN

    , corresponding to amino acids 145-165 of Ndufs1

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab52690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32.2 kDa (predicted molecular weight: 79 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
  • Involvement in diseaseDefects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similaritiesBelongs to the complex I 75 kDa subunit family.
    Contains 1 2Fe-2S ferredoxin-type domain.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI-75kD antibody
    • Complex I 75Kd antibody
    • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
    • Complex I-75kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
    • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
    • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
    • NDUFS1 antibody
    • NDUS1_HUMAN antibody
    • PRO1304 antibody
    see all

Anti-Ndufs1 antibody images

  • All lanes : Anti-Ndufs1 antibody (ab52690) at 1/1000 dilution

    Lane 1 : The negative control lane: ~20ug of a total protein extract from E coli ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
    Lane 2 : Test lane: ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 79 kDa
    Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

References for Anti-Ndufs1 antibody (ab52690)

ab52690 has not yet been referenced specifically in any publications.

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