Overview

  • Product name
    Anti-NDUFS2 antibody [7A12BE5AD5]
    See all NDUFS2 primary antibodies
  • Description
    Mouse monoclonal [7A12BE5AD5] to NDUFS2
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human heart mitochondria (UniProt ID: O75306).

  • Positive control
    • WB: Isolated mitochondria from Human liver, Bovine heart, H9C2 cells (Rat cardiomyocyte) and MEF cells (Mouse embryo fibroblast) ICC/IF: HDFn cultured cells (normal Human dermal fibroblasts, neonatal)
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities
    Belongs to the complex I 49 kDa subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 49 antibody
    • CI 49kD antibody
    • CI-49kD antibody
    • Complex 1, mitochondrial respiratory chain, 49 KD subunit antibody
    • Complex I 49kD antibody
    • Complex I 49kDa subunit antibody
    • Complex I-49kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibody
    • NADH ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH ubiquinone oxidoreductase NDUFS2 subunit antibody
    • NADH-ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH:ubiquinone oxidoreductase core subunit S2 antibody
    • Ndufs2 antibody
    • NDUS2_HUMAN antibody
    see all

Anti-NDUFS2 antibody [7A12BE5AD5] images



  • Predicted band size : 53 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab110249 observed at 48 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab110249 was shown to specifically react with NDUFS2 in wild type cells as signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab110249 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 0.25 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Overlay histogram showing HepG2 cells stained with ab110249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110249, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249) at 0.25 µg/ml

    Lane 1 : Molecular Weight Markers
    Lane 2 : Isolated mitochondria from Human liver
    Lane 3 : Isolated mitochondria from Bovine heart
    Lane 4 : Isolated mitochondria from H9C2 cells (Rat cardiomyocyte)
    Lane 5 : Isolated mitochondria from MEF cells (Mouse embryo fibroblast)
    Lane 6 : Isolated mitochondria from HepG2


    Predicted band size : 53 kDa
    Complex I-subunit NDUFS2 is detected in the Human samples with Human specific ab110249, while Complex I-subunit NDUFB8 is detected in all samples (Human, Mouse and Rat) with an anti-NDUFB8.
  • Mitochondrial localization of Complex I subunit NDUFS2 visualized by Immunocytochemistry in HDFn cultured cells (normal Human dermal fibroblasts, neonatal), using ab110249 at 5 µg/ml.

References for Anti-NDUFS2 antibody [7A12BE5AD5] (ab110249)

This product has been referenced in:
  • Hernansanz-Agustín P  et al. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. Redox Biol 12:1040-1051 (2017). WB . Read more (PubMed: 28511347) »
  • Zurita Rendón O  et al. A Mutation in the Flavin Adenine Dinucleotide-Dependent Oxidoreductase FOXRED1 Results in Cell-Type-Specific Assembly Defects in Oxidative Phosphorylation Complexes I and II. Mol Cell Biol 36:2132-40 (2016). Read more (PubMed: 27215383) »

See all 5 Publications for this product

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