Overview

  • Product name
  • Description
    Rabbit polyclonal to NDUFS2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human NDUFS2 aa 200-430. The exact sequence is proprietary.
    Sequence:

    F FWLFEEREKM FEFYERVSGA RMHAAYIRPG GVHQDLPLGL MDDIYQFSKN FSLRLDELEE LLTNNRIWRN RTIDIGVVTA EEALNYGFSG VMLRGSGIQW DLRKTQPYDV YDQVEFDVPV GSRGDCYDRY LCRVEEMRQS LRIIAQCLNK MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA


    Database link: O75306

  • Positive control
    • Human kidney tissue; HeLa cells; 293T, A431, Jurkat, Raji cell lysate, Mouse brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab192764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 7.5 µg/ml.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in disease
    Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities
    Belongs to the complex I 49 kDa subunit family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 49 antibody
    • CI 49kD antibody
    • CI-49kD antibody
    • Complex 1, mitochondrial respiratory chain, 49 KD subunit antibody
    • Complex I 49kD antibody
    • Complex I 49kDa subunit antibody
    • Complex I-49kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibody
    • NADH ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH ubiquinone oxidoreductase NDUFS2 subunit antibody
    • NADH-ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH:ubiquinone oxidoreductase core subunit S2 antibody
    • Ndufs2 antibody
    • NDUS2_HUMAN antibody
    see all

Images

  • All lanes : Anti-NDUFS2 antibody (ab192764) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 53 kDa
  • Anti-NDUFS2 antibody (ab192764) at 1/10000 dilution + Mouse brain tissue lysate at 50 µg

    Predicted band size : 53 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling NDUFS2 with ab192764 at 7.5μg/mL.

  • Immunofluorescent analysis of HeLa cells labeling mitochondrial NDUFS2 with ab192764 at a 1/500 dilution. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. The panel on the right shows the nuclear stain.

References

ab192764 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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