Overview

  • Product nameAnti-NDUFS2 antibody
    See all NDUFS2 primary antibodies
  • Description
    Mouse polyclonal to NDUFS2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Goat, Cow
  • Immunogen

    Full length human NDUFS2 protein (NP_004541.1)

  • Positive control
    • NDUFS2 transfected 293T cell lysate, human kidney lysate

Applications

Our Abpromise guarantee covers the use of ab70408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in diseaseDefects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similaritiesBelongs to the complex I 49 kDa subunit family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 49 antibody
    • CI 49kD antibody
    • CI-49kD antibody
    • Complex 1, mitochondrial respiratory chain, 49 KD subunit antibody
    • Complex I 49kD antibody
    • Complex I 49kDa subunit antibody
    • Complex I-49kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2 49kDa antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 2, 49kDa (NADH coenzyme Q reductase) antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibody
    • NADH ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH ubiquinone oxidoreductase NDUFS2 subunit antibody
    • NADH-ubiquinone oxidoreductase 49 kDa subunit antibody
    • NADH:ubiquinone oxidoreductase core subunit S2 antibody
    • Ndufs2 antibody
    • NDUS2_HUMAN antibody
    see all

Anti-NDUFS2 antibody images

  • Anti-NDUFS2 antibody (ab70408) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-NDUFS2 antibody (ab70408) at 1/500 dilution

    Lane 1 : NDUFS2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-NDUFS2 antibody (ab70408) at 1/1000 dilution

    Lane 1 : Mouse endometrial whole cell lysate at 20 µg
    Lane 2 : Mouse 3T3L-1 whole cell lysate at 20 µg
    Lane 3 : Goat MG whole cell lysate at 30 µg
    Lane 4 : Bovine MAC-T whole cell lysate at 30 µg

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is a courtesy of YongCheng Jin

    See Abreview

References for Anti-NDUFS2 antibody (ab70408)

ab70408 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (cell type)
Loading amount 20 µg
Specification cell type
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. YongCheng Jin

Verified customer

Submitted Feb 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"