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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFS3 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O75489
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab177471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
Image is courtesy of an anonymous Abreview.
Immunofluorescence analysis of 293 cells labeling NDUFS3 with ab177471 at 1/50 dilution (green). DAPI nuclear staining (blue).
Flow cytometry analysis of HEK293 (Human embryonic kidney epithelial cell) cells labeling NDUFS3 (red) with purified ab177471 at a 1/200 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1XPBS (negative control) using ab177471at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemcal analysis of paraffin-embedded Human heart tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
Immunohistochemcal analysis of paraffin-embedded Human kidney tissue labeling NDUFS3 with ab177471 at 1/50 dilution.
ab177471 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"