Overview

  • Product nameAnti-Ndufs4 antibody
    See all Ndufs4 primary antibodies
  • Description
    Mouse monoclonal to Ndufs4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 66-176 of Human Ndufs4

Associated products

Applications

Our Abpromise guarantee covers the use of ab55540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in diseaseDefects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similaritiesBelongs to the complex I NDUFS4 subunit family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQDQ antibody
    • CI 18 antibody
    • CI 18 kDa antibody
    • CI AQDQ antibody
    • CI-18 kDa antibody
    • CI-AQDQ antibody
    • Complex I 18 kDa antibody
    • Complex I AQDQ antibody
    • Complex I-18 kDa antibody
    • Complex I-AQDQ antibody
    • mitochondrial antibody
    • mitochondrial respiratory chain complex I (18 KD subunit) antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
    • NADH dehydrogenase antibody
    • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
    • NDUFS4 antibody
    • NDUS4_HUMAN antibody
    see all

Anti-Ndufs4 antibody images



  • Predicted band size : 20 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Ndufs4knockout HAP1 cell lysate (20 µg)
    Lane 3: HEK293 cell lysate (20 µg)
    Lane 4: Hu fetal heart tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55540 observed at 28 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab55540 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. ab55540 and ab181602 (loading control to GAPDH) at a concentration of 1 µg/ml and a dilution of 1/10000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Ndufs4 antibody (ab55540) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 20 kDa
    Ndufs4 antibody (ab55540) at 1ug/lane + A-431 cell lysate at 25ug/lane.

References for Anti-Ndufs4 antibody (ab55540)

This product has been referenced in:
  • Charan RA  et al. Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin. Cell Death Dis 5:e1313 (2014). WB . Read more (PubMed: 24991765) »
  • Haack TB  et al. ELAC2 Mutations Cause a Mitochondrial RNA Processing Defect Associated with Hypertrophic Cardiomyopathy. Am J Hum Genet N/A:N/A (2013). WB . Read more (PubMed: 23849775) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"