Recombinant
RabMAb

Anti-NDUFS8 antibody [EPR11892] (ab170936)

Overview

  • Product name
    Anti-NDUFS8 antibody [EPR11892]
    See all NDUFS8 primary antibodies
  • Description
    Rabbit monoclonal [EPR11892] to NDUFS8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NDUFS8 aa 1-100. The exact sequence is proprietary.
    Database link: O00217

  • Positive control
    • HeLa, HepG2 and Jurkat cell lysates, Human liver tissue, Human thyroid carcinoma tissue, permeabilized Jurkat cells, immunoprecipitation pellet from HeLa cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab170936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.
  • Involvement in disease
    Defects in NDUFS8 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
  • Sequence similarities
    Belongs to the complex I 23 kDa subunit family.
    Contains 2 4Fe-4S ferredoxin-type domains.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 23kD antibody
    • CI-23kD antibody
    • Complex I 23kD antibody
    • Complex I-23kD antibody
    • EC 1.6.5.3 antibody
    • EC 1.6.99.3 antibody
    • Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8 antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody
    • NADH ubiquinone oxidoreductase 23 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 23 kDa subunit antibody
    • NDUFS8 antibody
    • NDUS8_HUMAN antibody
    • TYKY antibody
    • TYKY subunit antibody
    • Ubiquinoneiron sulfur protein 8, mitochondrial precursor antibody
    see all

Images

  • All lanes : Anti-NDUFS8 antibody [EPR11892] (ab170936) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : HepG2 cell lysates
    Lane 3 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NDUFS8 with ab170936 at 1/250 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling NDUFS8 with ab170936 at 1/250 dilution.

  • Flow cytometric analysis of permeabilized Jurkat cells using ab170936 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1X PBS (negative control) using ab170936 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab170936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170936.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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