Recombinant Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11893] to NDUFS8 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NDUFS8 antibody [EPR11893] - C-terminal
See all NDUFS8 primary antibodies -
Description
Rabbit monoclonal [EPR11893] to NDUFS8 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal heart, HepG2, Jurkat and HeLa lysates; Human endometrial carcinoma, Human heart and Human kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11893 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 23 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone. -
Involvement in disease
Defects in NDUFS8 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. -
Sequence similarities
Belongs to the complex I 23 kDa subunit family.
Contains 2 4Fe-4S ferredoxin-type domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 4728 Human
- Entrez Gene: 225887 Mouse
- Entrez Gene: 293652 Rat
- Omim: 602141 Human
- SwissProt: O00217 Human
- SwissProt: Q8K3J1 Mouse
- Unigene: 90443 Human
- Unigene: 44227 Mouse
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Alternative names
- CI 23kD antibody
- CI-23kD antibody
- Complex I 23kD antibody
see all
Images
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All lanes : Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183) at 1/1000 dilution
Lane 1 : Fetal heart tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling NDUFS8 with ab180183 at 1/10 (red) or a rabbit IgG negative (green).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab180183 has been referenced in 3 publications.
- Yoval-Sánchez B et al. Redox-dependent loss of flavin by mitochondria complex I is different in brain and heart. Redox Biol 51:102258 (2022). PubMed: 35189550
- Ansari F et al. Quantification of NADH:ubiquinone oxidoreductase (complex I) content in biological samples. J Biol Chem 297:101204 (2021). PubMed: 34543622
- Xu W et al. Sodium Benzoate Attenuates Secondary Brain Injury by Inhibiting Neuronal Apoptosis and Reducing Mitochondria-Mediated Oxidative Stress in a Rat Model of Intracerebral Hemorrhage: Possible Involvement of DJ-1/Akt/IKK/NF?B Pathway. Front Mol Neurosci 12:105 (2019). PubMed: 31114478