Recombinant
RabMAb

Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183)

Overview

  • Product name
    Anti-NDUFS8 antibody [EPR11893] - C-terminal
    See all NDUFS8 primary antibodies
  • Description
    Rabbit monoclonal [EPR11893] to NDUFS8 - C-terminal
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFS8 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O00217

  • Positive control
    • Fetal heart, HepG2, Jurkat and HeLa lysates; Human endometrial carcinoma, Human heart and Human kidney tissues; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab180183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.
    • Involvement in disease
      Defects in NDUFS8 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    • Sequence similarities
      Belongs to the complex I 23 kDa subunit family.
      Contains 2 4Fe-4S ferredoxin-type domains.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • CI 23kD antibody
      • CI-23kD antibody
      • Complex I 23kD antibody
      • Complex I-23kD antibody
      • EC 1.6.5.3 antibody
      • EC 1.6.99.3 antibody
      • Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8 antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody
      • NADH ubiquinone oxidoreductase 23 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 23 kDa subunit antibody
      • NDUFS8 antibody
      • NDUS8_HUMAN antibody
      • TYKY antibody
      • TYKY subunit antibody
      • Ubiquinoneiron sulfur protein 8, mitochondrial precursor antibody
      see all

    Images

    • Flow Cytometrical analysis of permeabilized HeLa cells labeling NDUFS8 with ab180183 at 1/10 (red) or a rabbit IgG negative (green).

    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling NDUFS8 with ab180183 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS8 with ab180183 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue labeling NDUFS8 with ab180183 at 1/50.

    • All lanes : Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 23 kDa

    References

    ab180183 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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