Overview

  • Product nameAnti-NDUFV1 antibody
    See all NDUFV1 primary antibodies
  • Description
    Mouse monoclonal to NDUFV1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KAIARLIEFY KHESCGQCTP CREGVDWMNK VMARFVRGDA RPAEIDSLWE ISKQIEGHTI CALGDGAAWP VQGLIRHFRP ELEERMQRFA QQHQARQAAS , corresponding to amino acids 365-465 of Human NDUFV1

Applications

Our Abpromise guarantee covers the use of ab55535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Involvement in diseaseDefects in NDUFV1 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in NDUFV1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similaritiesBelongs to the complex I 51 kDa subunit family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CI 51kD antibody
    • CI-51kD antibody
    • Complex I 51kD antibody
    • Complex I-51kD antibody
    • FLJ59059 antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) flavoprotein 1 antibody
    • NADH dehydrogenase [ubiquinone] flavoprotein 1 antibody
    • NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial antibody
    • NADH dehydrogenase flavoprotein 1 antibody
    • NADH ubiquinone oxidoreductase 51 kDa subunit antibody
    • NADH ubiquinone oxidoreductase antibody
    • NADH-ubiquinone oxidoreductase 51 kDa subunit antibody
    • NDUFV 1 antibody
    • ndufv1 antibody
    • NDUV1_HUMAN antibody
    • UQOR1 antibody
    see all

Anti-NDUFV1 antibody images

  • Overlay histogram showing HepG2 cells stained with ab55535 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55535, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


  • Predicted band size : 51 kDa
    NDUFV1 antibody (ab55535) at 1ug/lane + A-431 cell lysate at 25ug/lane.

References for Anti-NDUFV1 antibody (ab55535)

ab55535 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"