Predicted to react with Human rotaviruses (91% identity with immunogen) due to sequence homology.
Bovine Nebraska Calf Diarrhoea Virus.
For detection of bovine and human rotaviruses.
Purified IgG fraction of antiserum covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is not present. Estimated Molar HRP: IgG substitution is 2 to 3.
Shipped at 4°C. Store at +4°C.
Preservative: 0.002% Thimerosal (merthiolate).
Do not use sodium azide as a preservative as this will substantially inhibit the enzyme activity of horseradish peroxidase.
Constituents: PBS, 10mg/ml BSA
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Bovine virus diarrhea (BVD) is a complicated disease to discuss as it can result in a wide variety of disease problems from very mild to very severe. BVD can be one of the most devastating diseases cattle encounter and one of the hardest to get rid of when it attacks a herd. The viruses that cause BVD have been grouped into two genotypes, Type I and Type II. The disease syndrome caused by the two genotypes is basically the same, however disease caused by Type II infection is often more severe. The various disease syndromes noted in cattle infected with BVD virus are mainly attributed to the age of the animal when it became infected and to certain characteristics of the virus involved.