Overview

  • Product nameAnti-NECAP1 antibody
    See all NECAP1 primary antibodies
  • Description
    Rabbit polyclonal to NECAP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 90-139 (RYFVIRIQDG TGRSAFIGIG FTDRGDAFDF NVSLQDHFKW VKQETEISKE) of Mouse NECAP1 protein (NP_080543).

  • Positive control
    • Mouse spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab112090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in endocytosis.
  • Sequence similaritiesBelongs to the NECAP family.
  • DomainThe WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2.
  • Cellular localizationCytoplasmic vesicle > clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adaptin ear binding coat associated protein 1 antibody
    • Adaptin ear-binding coat-associated protein 1 antibody
    • DKFZP566B183 antibody
    • MGC131900 antibody
    • NECAP 1 antibody
    • NECAP endocytosis associated 1 antibody
    • NECAP endocytosis-associated protein 1 antibody
    • NECAP-1 antibody
    • NECAP1 antibody
    • NECP1_HUMAN antibody
    see all

Anti-NECAP1 antibody images

  • Anti-NECAP1 antibody (ab112090) at 1 µg/ml + Mouse spleen lysate at 10 µg

    Predicted band size : 30 kDa

References for Anti-NECAP1 antibody (ab112090)

ab112090 has not yet been referenced specifically in any publications.

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