Overview

  • Product nameAnti-NECAP2 antibody
    See all NECAP2 primary antibodies
  • Description
    Mouse polyclonal to NECAP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-263 of Human NECAP2

  • Positive control
    • NECAP2 transfected 293T cell lysate; human stomach tissue lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects bands of approximately 37 and 31 kDa in separate blots(predicted molecular weight: 28 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in endocytosis.
    • Sequence similaritiesBelongs to the NECAP family.
    • DomainThe WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
    • Cellular localizationCytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    Anti-NECAP2 antibody images

    • All lanes : Anti-NECAP2 antibody (ab68747) at 1/500 dilution

      Lane 1 : NECAP2 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • Anti-NECAP2 antibody (ab68747) at 1/500 dilution + NECAP2 expressed in human stomach at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-NECAP2 antibody (ab68747)

    ab68747 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68747.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"