Overview

  • Product nameAnti-Necdin antibody
    See all Necdin primary antibodies
  • Description
    Mouse monoclonal to Necdin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: WKKHSTFGDV RKLITEEFVQ MNYLKYQRVP YVEPPEYEFF WGSRASREIT KMQIMEFLAR VFKKDPQAWP SRYREALEEA RALREANPTA HYPRSSVSED , corresponding to amino acids 222-322 of Human Necdin

Properties

Applications

Our Abpromise guarantee covers the use of ab55501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGrowth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
    • Tissue specificityAlmost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
    • Sequence similaritiesContains 1 MAGE domain.
    • Cellular localizationPerikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • HsT16328 antibody
      • NDN antibody
      • NECD_HUMAN antibody
      • Necdin antibody
      • Necdin homolog antibody
      • necdin related protein antibody
      • PWCR antibody
      see all

    Anti-Necdin antibody images

    • Necdin antibody (ab55501) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.


    • Predicted band size : 36 kDa
      Necdin antibody (ab55501) at 1ug/lane + HL-60 cell lysate at 25ug/lane.

    References for Anti-Necdin antibody (ab55501)

    ab55501 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab55501.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"