Overview

  • Product nameAnti-Necdin antibody
    See all Necdin primary antibodies
  • Description
    Rabbit polyclonal to Necdin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 180-229 (ALSNRMPMTG LLLMILSLIY VKGRGARESA VWNVLRILGL RPWKKHSTFG) of Human Necdin (NP_002478).

  • Positive control
    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionGrowth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • Tissue specificityAlmost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
  • Sequence similaritiesContains 1 MAGE domain.
  • Cellular localizationPerikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT16328 antibody
    • NDN antibody
    • NECD_HUMAN antibody
    • Necdin antibody
    • Necdin homolog antibody
    • necdin related protein antibody
    • PWCR antibody
    see all

Anti-Necdin antibody images

  • Anti-Necdin antibody (ab94529) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

References for Anti-Necdin antibody (ab94529)

ab94529 has not yet been referenced specifically in any publications.

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