Overview

  • Product name
    Anti-Nectin 2 antibody [B-C12]
    See all Nectin 2 primary antibodies
  • Description
    Mouse monoclonal [B-C12] to Nectin 2
  • Specificity
    ab47171 recognises the Nectin-2, the polio retrovirus receptor-2 (PRR2)
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KG-1 cell line

  • Positive control
    • KG-1 cell line

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free
  • Clonality
    Monoclonal
  • Clone number
    B-C12
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
ICC/IF
Flow Cyt
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1-10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable cell adhesion protein.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 112 antibody
      • CD112 antibody
      • CD112 antigen antibody
      • Herpes virus entry mediator B antibody
      • Herpes virus entry protein B antibody
      • Herpesvirus entry mediator B antibody
      • Herpesvirus entry protein B antibody
      • Hve B antibody
      • HveB antibody
      • Nectin-2 antibody
      • Nectin2 antibody
      • Poliovirus receptor like 2 antibody
      • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
      • Poliovirus receptor related 2 antibody
      • Poliovirus receptor related protein 2 antibody
      • Poliovirus receptor-related protein 2 antibody
      • PRR 2 antibody
      • PRR2 antibody
      • PVRL 2 antibody
      • PVRL2 antibody
      • PVRL2_HUMAN antibody
      • PVRR 2 antibody
      • PVRR2 antibody
      see all

    Images

    • A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line
    • ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Ogbomo H  et al. Myxoma virus infection promotes NK lysis of malignant gliomas in vitro and in vivo. PLoS One 8:e66825 (2013). Flow Cyt ; Human . Read more (PubMed: 23762498) »

    See 1 Publication for this product

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    There are currently no Customer reviews or Questions for ab47171.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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