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Mouse monoclonal [B-C12] to Nectin 2
ab47171 recognises the Nectin-2, the polio retrovirus receptor-2 (PRR2)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Ion Exchange Chromatography
Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1-10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Anti-Nectin 2 antibody [B-C12] images
Flow Cytometry / FACS - Nectin 2 antibody [B-C12] - Azide free (ab47171)
A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line
Immunocytochemistry/ Immunofluorescence - Nectin 2 antibody [B-C12] - Azide free (ab47171)
ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"