Recombinant Anti-Nectin 2 antibody [EPR6717] (ab135246)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6717] to Nectin 2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nectin 2 antibody [EPR6717]
See all Nectin 2 primary antibodies -
Description
Rabbit monoclonal [EPR6717] to Nectin 2 -
Host species
Rabbit -
Specificity
ab135246 does not recognize Isoform Alpha of human Nectin-2. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Nectin 2 aa 500-600 (intracellular). The exact sequence is proprietary.
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Positive control
- Flow Cyt (intra): HUVEC cells; ICC/IF: HT-29 cells; WB: HT-29 and SW480 cells, mouse and rat brain lysate; IP: SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6717 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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ICC/IF |
1/50.
For unpurified use at 1/250 - 1/500 |
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IP |
1/20.
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Notes |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
ICC/IF
1/50. For unpurified use at 1/250 - 1/500 |
IP
1/20. |
Target
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Function
Probable cell adhesion protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5819 Human
- Entrez Gene: 19294 Mouse
- Entrez Gene: 308417 Rat
- Omim: 600798 Human
- SwissProt: Q92692 Human
- SwissProt: P32507 Mouse
- Unigene: 655455 Human
- Unigene: 4341 Mouse
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Alternative names
- CD 112 antibody
- CD112 antibody
- CD112 antigen antibody
see all
Images
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Purified ab135246 at 1/20 dilution (1µg) immunoprecipitating Nectin 2 in SW480 whole cell lysate.
Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab135246 + SW480 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab135246 in SW480 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70-85 kDa -
Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70-85 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).
Blocking Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilution (Purified)
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70-85 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15108322, PMID:12445825 and PMID:12913096).
Blocking Buffer and concentration: 5% NFDM/TBST
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Immunocytochemistry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Nectin 2 with Purified ab135246 at 1:50 dilution (3.06 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling Nectin 2 with Purified ab135246 at 1/20 dilution (10µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab135246 has been referenced in 12 publications.
- Yang HY et al. Gelsolin impairs barrier function in pancreatic ductal epithelial cells by actin filament depolymerization in hypertriglyceridemia-induced pancreatitis in vitro. Exp Ther Med 23:290 (2022). PubMed: 35317441
- Nishimura T et al. Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells. Dev Cell 56:842-859.e8 (2021). PubMed: 33756122
- Hu HT et al. Ultracentrifugal separation, characterization, and functional study of extracellular vesicles derived from serum-free cell culture. STAR Protoc 2:100625 (2021). PubMed: 34223199
- Xu K et al. Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis. Oncogenesis 10:66 (2021). PubMed: 34611125
- Wang C et al. Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury. Mucosal Immunol 13:22-33 (2020). PubMed: 31719643