Overview

  • Product name
    Anti-Nectin 3 antibody [103-A1]
    See all Nectin 3 primary antibodies
  • Description
    Rat monoclonal [103-A1] to Nectin 3
  • Tested applications
    Suitable for: Functional Studies, ICC/IF, Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400 fused to IgG Fc.

  • Positive control
    • L-cells (A cultured line of C3H mouse fibroblasts)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Provided as a 0.2µm purified antibody solution
  • Clonality
    Monoclonal
  • Clone number
    103-A1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
    • Tissue specificity
      Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
    • Sequence similarities
      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD113 antibody
      • CDw113 antibody
      • DKFZP566B0846 antibody
      • FLJ90624 antibody
      • Nectin-3 antibody
      • Nectin3 antibody
      • Poliovirus receptor related 3 antibody
      • Poliovirus receptor related protein 3 antibody
      • Poliovirus receptor-like 3 antibody
      • Poliovirus receptor-related protein 3 antibody
      • PPR3 antibody
      • PRR3 antibody
      • Pvrl3 antibody
      • PVRL3_HUMAN antibody
      • PVRR3 antibody
      see all

    References

    This product has been referenced in:
    • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet 131:235-50 (2012). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »
    • Tanaka-Okamoto M  et al. Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. Cancer Sci 100:608-16 (2009). Read more (PubMed: 19215226) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting Abcam.

    According to our notes, ab16913 is not suitable for western blotting. However the Nectin3 antibodies listed below, are suitable for use in western blotting:

    http://www.abcam.com/Nectin-3-antibody-ab6393...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 10 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsination
    Sample buffer: PBS, 4%FCS
    Permeabilization
    No
    Gating Strategy
    Debris was gated away by FSC/SSC and dead cells by PI.
    Username

    Abcam user community

    Verified customer

    Submitted May 09 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Specification
    testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Y Toyama

    Verified customer

    Submitted Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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