Overview

  • Product nameAnti-Nectin 3 antibody
    See all Nectin 3 primary antibodies
  • Description
    Rabbit polyclonal to Nectin 3
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to a region surrounding amino acid 536 of mouse nectin 3

  • Positive control
    • 3T3 cell lysate; Rat kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab63931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).

Target

  • FunctionPlays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
  • Tissue specificityPredominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
  • Sequence similaritiesBelongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD113 antibody
    • CDw113 antibody
    • DKFZP566B0846 antibody
    • FLJ90624 antibody
    • Nectin-3 antibody
    • Nectin3 antibody
    • Poliovirus receptor related 3 antibody
    • Poliovirus receptor related protein 3 antibody
    • Poliovirus receptor-like 3 antibody
    • Poliovirus receptor-related protein 3 antibody
    • PPR3 antibody
    • PRR3 antibody
    • Pvrl3 antibody
    • PVRL3_HUMAN antibody
    • PVRR3 antibody
    see all

Anti-Nectin 3 antibody images

  • All lanes : Anti-Nectin 3 antibody (ab63931) at 1 µg

    Lane 1 : Rat kidney lysate
    Lane 2 : 3T3 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa

References for Anti-Nectin 3 antibody (ab63931)

This product has been referenced in:
  • Luaces JP  et al. Loss of Sertoli-germ cell adhesion determines the rapid germ cell elimination during the seasonal regression of the seminiferous epithelium of the large hairy armadillo Chaetophractus villosus. Biol Reprod 90:48 (2014). Read more (PubMed: 24451984) »
  • van der Kooij MA  et al. Role for MMP-9 in stress-induced downregulation of nectin-3 in hippocampal CA1 and associated behavioural alterations. Nat Commun 5:4995 (2014). WB ; Rat . Read more (PubMed: 25232752) »

See all 8 Publications for this product

Product Wall

We have previously been in contact regarding ab63931, which was not providing satisfactory results.

We provided some troubleshooting tips for the protocol, and I am writing to inquire if these suggestions have helped to improve the results...

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Thank you for your reply.

What I wanted to say is that the HEK293 cells do also have Nectin-3 expression. The antibody ab63931 is likely to recognize also the Nectin-3 isoforms expressed by the human cell. Therefore it could be that the obser...

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Thank you for providing these additional details and your patience.

The laboratory has confirmed that the immunogen for thisantibody isa synthetic peptide corresponding to residues surrounding amino acids 536 of mouse Nectin-3 and that this r...

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Thank you for your patience.

Unfortunately we can not provide the exact immunogen sequence for this antibody as it is proprietary information of the laboratory.

As said, please provide us with more information about the experiment. ...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I have contacted the laboratory re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole C57BL/6 mouse hippocampus)
Loading amount 40 µg
Specification Whole C57BL/6 mouse hippocampus
Gel Running Conditions Non-reduced Denaturing (NuPAGE® 10% Bis-Tris gel (Invitrogen))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Hippocampus)
Specification Hippocampus
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"