Overview

  • Product name
    Anti-NEDD1 antibody
  • Description
    Goat polyclonal to NEDD1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-NQTRNSEKFEKPEN

    , corresponding to internal sequence amino acids 520-533 of Human NEDD1 (NP_690869.1).

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab56317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
    An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
    • Sequence similarities
      Contains 8 WD repeats.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ35902 antibody
      • GCP WD antibody
      • NEDD-1 antibody
      • NEDD1 antibody
      • NEDD1_HUMAN antibody
      • Neural precursor cell expressed developmentally down-regulated protein 1 antibody
      • Neural precursor cell expressed, developmentally down regulated gene 1 antibody
      • Protein NEDD1 antibody
      • TUBGCP7 antibody
      see all

    Images

    • Anti-NEDD1 antibody (ab56317) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 72 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References

    ab56317 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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