Overview

  • Product nameAnti-NEDD1 antibody
    See all NEDD1 primary antibodies
  • Description
    Goat polyclonal to NEDD1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-NQTRNSEKFEKPEN

    , corresponding to internal sequence amino acids 520-533 of Human NEDD1 (NP_690869.1).

  • Positive control
    • Hela cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
    An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for mitosis progression. Promotes the nucleation of microtubules from the spindle.
    • Sequence similaritiesContains 8 WD repeats.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ35902 antibody
      • GCP WD antibody
      • NEDD-1 antibody
      • NEDD1 antibody
      • NEDD1_HUMAN antibody
      • Neural precursor cell expressed developmentally down-regulated protein 1 antibody
      • Neural precursor cell expressed, developmentally down regulated gene 1 antibody
      • Protein NEDD1 antibody
      • TUBGCP7 antibody
      see all

    Anti-NEDD1 antibody images

    • Anti-NEDD1 antibody (ab56317) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 72 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-NEDD1 antibody (ab56317)

    ab56317 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab56317.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"