Overview

  • Product nameAnti-NEDD1 antibody
    See all NEDD1 primary antibodies
  • Description
    Mouse monoclonal to NEDD1
  • SpecificityThe immunogen used for ab57336 has ~80% homology with the X. laevis homologue of NEDD1. Some customers have successfully used ab57336 with X. laevis. Please see abreviews for more details.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Recombinant fragment: AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF , corresponding to amino acids 561-661 of Human NEDD1

Properties

Applications

Our Abpromise guarantee covers the use of ab57336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 22261722

Target

Anti-NEDD1 antibody images

  • Overlay histogram showing HeLa cells stained with ab57336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57336, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • NEDD1 antibody (ab57336) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.


  • Predicted band size : 72 kDa
    NEDD1 antibody (ab57336) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Anti-NEDD1 antibody (ab57336) staining NEDD1 in immunocytochemistry/immunofluorescence in Xenopus laevis cytoplasmic egg extracts. The localization by IF is what would be expected, at spindle pole and microtubules.

    See Abreview

References for Anti-NEDD1 antibody (ab57336)

This product has been referenced in:
  • Kim S & Rhee K Importance of the CEP215-pericentrin interaction for centrosome maturation during mitosis. PLoS One 9:e87016 (2014). WB ; Human . Read more (PubMed: 24466316) »
  • Al-Hakim AK  et al. Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture. Mol Cell Proteomics 11:M111.014233 (2012). ICC/IF ; Human . Read more (PubMed: 22261722) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification Spindles in X. laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3%
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Verified customer

Submitted Oct 09 2012

Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount 50 µg
Specification Xenopus laevis cytoplasmic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"