Overview

  • Product nameAnti-NEDD4-2 antibody
    See all NEDD4-2 primary antibodies
  • Description
    Rabbit polyclonal to NEDD4-2
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human NEDD4-2 (NP_001138439.1).

  • Positive control
    • Human prostate carcinoma; Mouse teratoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab124643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • Tissue specificityUbiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • Post-translational
    modifications
    Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • Cellular localizationCytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

Anti-NEDD4-2 antibody images

  • ab124643, at 1/250 dilution, staining NEDD4-2 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry with DAB staining.
  • ab124643, at 1/250 dilution, staining NEDD4-2 in formalin-fixed, paraffin-embedded Mouse teratoma tissue by Immunohistochemistry with DAB staining.

References for Anti-NEDD4-2 antibody (ab124643)

ab124643 has not yet been referenced specifically in any publications.

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