Overview

  • Product nameAnti-NEDD4-2 antibody
    See all NEDD4-2 primary antibodies
  • Description
    Mouse monoclonal to NEDD4-2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MATGLGEPVY GLSEDEGESR ILRVKVVSGI DLAKKDIFGA SDPYVKLSLY VADENRELAL VQTKTIKKTL NPKWNEEFYF RVNPSNHRLL FEVFDENRLT , corresponding to amino acids 1-100 of Human NEDD4-2

Properties

Applications

Our Abpromise guarantee covers the use of ab58093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 19617352
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • Tissue specificityUbiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • Post-translational
    modifications
    Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • Cellular localizationCytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

Anti-NEDD4-2 antibody images

  • Western blot against tagged recombinant protein immunogen using ab58093 NEDD4-2 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • IHC image of ab58093 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58093, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NEDD4-2 antibody (ab58093)

This product has been referenced in:
  • Das S  et al. Pathological role of serum- and glucocorticoid-regulated kinase 1 in adverse ventricular remodeling. Circulation 126:2208-19 (2012). WB ; Mouse . Read more (PubMed: 23019294) »
  • Simonin A & Fuster D Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function. J Biol Chem 285:38293-303 (2010). WB ; Human . Read more (PubMed: 20855896) »

See all 3 Publications for this product

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Expiration date: June 10, 2012

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