Overview

  • Product nameAnti-NEDD4-2 antibody
    See all NEDD4-2 primary antibodies
  • Description
    Rabbit polyclonal to NEDD4-2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NEDD4-2.

    (Peptide available as ab73385.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue; HepG2 Whole Cell; RAW 264.7 Whole Cell; EBS E14 TG2A Day 5 Whole cell

Properties

Applications

Our Abpromise guarantee covers the use of ab73386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • Tissue specificityUbiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • Post-translational
    modifications
    Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • Cellular localizationCytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

Anti-NEDD4-2 antibody images

  • All lanes : Anti-NEDD4-2 antibody (ab73386) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
    Additional bands at : 44 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab73386 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73386, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NEDD4-2 antibody (ab73386)

This product has been referenced in:
  • Eylenstein A  et al. Stimulation of Ca2+-channel Orai1/STIM1 by serum- and glucocorticoid-inducible kinase 1 (SGK1). FASEB J 25:2012-21 (2011). WB . Read more (PubMed: 21385992) »

See 1 Publication for this product

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