Anti-NEDD4-2 (phospho S328) antibody (ab95399)
Key features and details
- Rabbit polyclonal to NEDD4-2 (phospho S328)
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-NEDD4-2 (phospho S328) antibody
See all NEDD4-2 primary antibodies -
Description
Rabbit polyclonal to NEDD4-2 (phospho S328) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse NEDD4-2 conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
Tissue specificity
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
Post-translational
modificationsPhosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
Cellular localization
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
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Database links
- Entrez Gene: 83814 Mouse
- SwissProt: Q8CFI0 Mouse
- Unigene: 98668 Mouse
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Alternative names
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab95399 has been referenced in 4 publications.
- Deftu AF et al. The Antidiabetic Drug Metformin Regulates Voltage-Gated Sodium Channel NaV1.7 via the Ubiquitin-Ligase NEDD4-2. eNeuro 9:N/A (2022). PubMed: 35131865
- Saha B et al. WNK1 is a chloride-stimulated scaffold that regulates mTORC2 activity and ion transport. J Cell Sci 135:N/A (2022). PubMed: 36373794
- Gleason CE et al. mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity. J Clin Invest 125:117-28 (2015). PubMed: 25415435
- Caohuy H et al. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ?F508-cystic fibrosis transmembrane conductance regulator (?F508-CFTR). J Biol Chem 289:35953-68 (2014). PubMed: 25384981