Rabbit monoclonal [EPR8269(2)] to NEDD4-2 (phospho S448)
WB, IPmore details Unsuitable for:
ICC/IF or IHC-P
Mouse, Rat, Human
Synthetic peptide corresponding to Human NEDD4-2 (phospho S448). Corresponding to residues surrounding Serine 448. Database link: Q96PU5
293T cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 110-135 kDa (predicted molecular weight: 112 kDa).
1/10 - 1/100.
Is unsuitable for ICC/IF or IHC-P.
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.
Detection of NEDD4-2 by Western Blot of Immunprecipitate. 293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)
All lanes : Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated Lane 2 : 293T cell lysate, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa Additional bands at: 110-135 kDa. We are unsure as to the identity of these extra bands.