Overview

  • Product name
  • Description
    Rabbit polyclonal to NEDD4
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 725-775 of Human NEDD4 according to AAI36606.1

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab117826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 4 WW domains.
    • Domain
      The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.
    • Post-translational
      modifications
      Auto-ubiquitinated.
    • Cellular localization
      Cytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 53 protein antibody
      • E3 ubiquitin protein ligase Nedd4 antibody
      • E3 ubiquitin-protein ligase NEDD4 antibody
      • HECT-type E3 ubiquitin transferase NEDD4 antibody
      • KIAA0093 antibody
      • NEDD 4 antibody
      • NEDD-4 antibody
      • Nedd4 antibody
      • NEDD4_HUMAN antibody
      • Neural precursor cell expressed developmentally down regulated 4 antibody
      • Neural precursor cell expressed developmentally down-regulated protein 4 antibody
      • neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase antibody
      • PIG53 antibody
      • receptor-potentiating factor 1 antibody
      • RPF1 antibody
      see all

    Images

    • Detection of Human NEDD4 in HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab117826 at 6 µg/mg of lysate. Subsequent WB detection was done using another anti Human NEDD4 antibody. Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab117826 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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