Overview

  • Product nameAnti-NEDD4 antibody
    See all NEDD4 primary antibodies
  • Description
    Rabbit polyclonal to NEDD4
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein:

    PVLLPTSSGL PPGWEEKQDD RGRSYYVDHN SKTTTWSKPT MQDDPRSKIP AHLRGKTPVD SNDLGPLP

    , corresponding to the WW2 domain, amino acids 395-462 of Rat NEDD4.

  • Positive control
    • Mouse kidney, lung, and distal colon sections. Cytoplasmic extracts from mouse kidney cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab14592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 115 kDa.

This antibody has been used to detected yeast (saccharomyces cerevisiae) Rsp-5 protein, a Nedd4 homologue. See Abreview.

ICC/IF Use at an assay dependent concentration. See Abreview.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • DomainThe WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localizationCytoplasm. Cell membrane. Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 53 protein antibody
    • E3 ubiquitin protein ligase Nedd4 antibody
    • E3 ubiquitin-protein ligase NEDD4 antibody
    • KIAA0093 antibody
    • NEDD 4 antibody
    • NEDD-4 antibody
    • Nedd4 antibody
    • NEDD4_HUMAN antibody
    • Neural precursor cell expressed developmentally down regulated 4 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 4 antibody
    see all

References for Anti-NEDD4 antibody (ab14592)

This product has been referenced in:
  • Lin A & Man HY Endocytic Adaptor Epidermal Growth Factor Receptor Substrate 15 (Eps15) Is Involved in the Trafficking of Ubiquitinated a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors. J Biol Chem 289:24652-64 (2014). WB ; Rat . Read more (PubMed: 25023288) »
  • MacNeil LG  et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . Read more (PubMed: 25289940) »

See all 11 Publications for this product

Product Wall

Thank you very much for your patience. The protein used as an immunogen was indeed a GST fusion, so cross-reaction with GST is to be expected. I hope this clears up the cross-reactivity issue you have been seeing! I hope this information helps, ...

Read More
Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing
Sample Human Cell lysate - whole cell (PC3)
Specification PC3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Wei Cui

Verified customer

Submitted Mar 11 2015

Thank you for your inquiry. Unfortunately our source of this antibody is not able to provide the blocking peptide for ab14592. If this is of any help, you might have seen that the sequence of the immunogen is available on the website. We do have...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Specification HEK293T
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Teresa Gunn

Verified customer

Submitted Nov 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 50 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Teresa Gunn

Verified customer

Submitted Sep 26 2007

Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Yeast extract)
Loading amount 1 µg
Specification Yeast extract
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4
Username

Abcam user community

Verified customer

Submitted Nov 20 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (liver)
Specification liver
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Miss. Komla-Soukha Isabel

Verified customer

Submitted Jan 05 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"