NEDD4 antibody (ab14592)

Product Name 

NEDD4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NEDD4

Immunogen 

Fusion protein: PVLLPTSSGL PPGWEEKQDD RGRSYYVDHN SKTTTWSKPT MQDDPRSKIP AHLRGKTPVD SNDLGPLP, corresponding to the WW2 domain, amino acids 395-462 of Rat NEDD4.
BLAST 'PVLLPTSSGL PPGWEEKQD D RGRSYYVD HN SKTTTWS KPT MQDDPR SKIP AHLRG KTPVD SNDL ' with or

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/5000 - 1/20000. Detects a band of approximately 115 kDa.

This antibody has been used to detected yeast(saccharomyces cerevisiae) Rsp-5 protein, a Nedd4 homologue. See Abreview.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse kidney, lung, and distal colon sections.
Cytoplasmic extracts from mouse kidney cells.

Cellular localization 

Cytoplasmic

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Neuroscience >> Neurology process >> Neurogenesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin

Relevance 

Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 5mg/ml BSA, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for NEDD4 antibody (ab14592)

  This product has been used in: (two most recent references)

Al Sorkhy M et al. The cyclin-dependent kinase activator, Spy1A, is targeted for degradation by the ubiquitin ligase NEDD4. J Biol Chem 284:2617-27 (2009). WB; Human. PubMed: 19054764

Kotorashvili A et al. Anterograde transport of surfactant protein C proprotein to distal processing compartments requires PPDY-mediated association with Nedd4 ubiquitin ligases. J Biol Chem 284:16667-78 (2009). WB; Human. PubMed: 19366705

See all 5 publication references for this product.


If you publish research using ab14592 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding NEDD4 antibody

Customer FAQs regarding NEDD4 antibody

Protocols for NEDD4 antibody

Price and availability of products related to NEDD4 antibody

NEDD4 antibody - related products: