• Product nameAnti-NEDD8 antibody
    See all NEDD8 primary antibodies
  • Description
    Rabbit polyclonal to NEDD8
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH-conjugated synthetic peptide selected within aa 1-100 of human NEDD8.

  • Positive control
    • HeLa cell lysates and human hepatocarcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab38634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 9 kDa.
IHC-P 1/50 - 1/100.


  • FunctionUbiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  • Tissue specificityHighly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • Sequence similaritiesBelongs to the ubiquitin family.
  • Post-translational
    Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
  • Cellular localizationNucleus. Mainly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ43224 antibody
    • MGC104393 antibody
    • MGC125896 antibody
    • MGC125897 antibody
    • NED8 antibody
    • NEDD 8 antibody
    • NEDD-8 antibody
    • Nedd8 antibody
    • NEDD8_HUMAN antibody
    • Neddylin antibody
    • Neural precursor cell expressed developmentally down regulated 8 antibody
    • Neural precursor cell expressed developmentally down regulated gene 8 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 8 antibody
    • Rub1 antibody
    • Ubiquitin like protein Nedd 8 antibody
    • Ubiquitin like protein Nedd8 antibody
    • Ubiquitin-like protein Nedd8 antibody
    see all

Anti-NEDD8 antibody images

  • Anti-NEDD8 antibody (ab38634) at 1/100 dilution + HeLa cell lysates

    Predicted band size : 9 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-NEDD8 antibody (ab38634)

This product has been referenced in:
  • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). Read more (PubMed: 22504483) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (mcf 7)
Specification mcf 7
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 37°C

Mr. Sanjib Chaudhary

Verified customer

Submitted Aug 12 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Jun 15 2010