Overview

  • Product name
  • Description
    Rabbit polyclonal to NEI3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the internal region of Human NEI3.

  • Positive control
    • COLO, HuvEc, HepG2 and HeLa cell extracts

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.

Target

  • Function
    Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.
  • Tissue specificity
    Detected in thymus and testis. Expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas and testis where expression is higher in normal than tumor tissue.
  • Sequence similarities
    Belongs to the FPG family.
    Contains 1 FPG-type zinc finger.
    Contains 1 RanBP2-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 123 antibody
    • AI449477 antibody
    • BC034753 antibody
    • C85903 antibody
    • DNA glycosylase FPG2 antibody
    • DNA glycosylase hFPG2 antibody
    • DNA glycosylase/AP lyase Neil3 antibody
    • EC 3.2.2.- antibody
    • EC 4.2.99.18 antibody
    • Endonuclease 8-like 3 antibody
    • Endonuclease VIII-like 3 antibody
    • FGP2 antibody
    • FLJ10858 antibody
    • FPG2 antibody
    • hFPG2 antibody
    • hNEI3 antibody
    • MGC27861 antibody
    • MGC36916 antibody
    • Nei endonuclease VIII-like 3 (E. coli) antibody
    • nei endonuclease VIII-like 3 antibody
    • Nei like 3 (E. coli) antibody
    • Nei-like protein 3 antibody
    • NEIL3 antibody
    • NEIL3_HUMAN antibody
    • OTTHUMP00000218810 antibody
    • RGD1310562 antibody
    see all

Images

  • All lanes : Anti-NEI3 antibody (ab110049) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : HuvEc cell extract
    Lane 3 : HepG2 cell extract
    Lane 4 : HeLa cell extract
    Lane 5 : HuvEc cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 68 kDa

References

ab110049 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab110049.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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