Overview

  • Product name
  • Description
    Rabbit polyclonal to NEIL1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 137-167 of Human NEIL1.

  • Positive control
    • A2058 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab103137 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 44 kDa.

Target

  • Function
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the FPG family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA (apurinic or apyrimidinic site) lyase Neil1 antibody
    • DNA glycosylase/AP lyase Neil 1 antibody
    • DNA glycosylase/AP lyase Neil1 antibody
    • DNA-(apurinic or apyrimidinic site) lyase Neil1 antibody
    • Endonuclease 8 like 1 antibody
    • Endonuclease 8-like 1 antibody
    • Endonuclease VIII antibody
    • Endonuclease VIII like 1 antibody
    • Endonuclease VIII-like 1 antibody
    • FLJ22402 antibody
    • FPG1 antibody
    • hFPG1 antibody
    • NEH 1 antibody
    • NEH1 antibody
    • NEI 1 antibody
    • Nei endonuclease VIII like 1 (E. coli) antibody
    • Nei endonuclease VIII like 1 antibody
    • Nei homolog 1 antibody
    • Nei like 1 antibody
    • Nei like protein 1 antibody
    • Nei-like protein 1 antibody
    • NEI1 antibody
    • NEIL 1 antibody
    • Neil1 antibody
    • NEIL1_HUMAN antibody
    see all

Anti-NEIL1 antibody images

  • Anti-NEIL1 antibody (ab103137) at 1/50 dilution + A2058 cell lysate at 35 µg

    Predicted band size : 44 kDa

References for Anti-NEIL1 antibody (ab103137)

ab103137 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - nuclear (lung)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
28 µg
Specification
lung
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Submitted Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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