Overview

  • Product nameAnti-NEK11 antibody
    See all NEK11 primary antibodies
  • Description
    Mouse monoclonal to NEK11
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-483 of Human NEK11

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPossible role in S-phase checkpoint mechanism.
    • Tissue specificityPoorly expressed in cerebellum, trachea, lung, appendix, and uterus.
    • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Developmental stagePredominantly expressed at S/G2/M phase.
    • Post-translational
      modifications
      Phosphorylated.
    • Cellular localizationNucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4932416N14Rik antibody
      • FLJ23495 antibody
      • NEK11 antibody
      • NEK11_HUMAN antibody
      • NEK11S/L antibody
      • Never in mitosis A-related kinase 11 antibody
      • NIMA (never in mitosis gene a) related kinase 11 antibody
      • NIMA (never in mitosis gene a)- related kinase 11 antibody
      • NIMA related kinase 11L antibody
      • NIMA related kinase 11S antibody
      • NIMA-related kinase 11 antibody
      • NimA-related protein kinase 11 antibody
      • Serine/threonine-protein kinase Nek11 antibody
      see all

    Anti-NEK11 antibody images

    • NEK11 antibody (ab57747) used in immunofluorescence at 10ug/ml on HeLa cells.
    • NEK11 antibody (ab57747) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium tissue.


    • Predicted band size : 74 kDa
      NEK11 antibody (ab57747) at 1ug/lane + HL-60 cell lysate at 25ug/lane.

    References for Anti-NEK11 antibody (ab57747)

    ab57747 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab57747.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"