Overview

  • Product nameAnti-NEK2 antibody
    See all NEK2 primary antibodies
  • Description
    Rabbit polyclonal to NEK2
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GRQLGEPEKSQDS

    conjugated to KLH, corresponding to amino acids 287-299 of Human NEK2

  • Positive control
    • Human testis tissue; Human colon tissue
  • General notesStore undiluted

Properties

Applications

Our Abpromise guarantee covers the use of ab115731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/2000 - 1/10000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionProtein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
  • Tissue specificityIsoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
  • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageAccumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
  • Post-translational
    modifications
    It is unsure whether Thr-170 or Ser-171 is phosphorylated.
  • Cellular localizationCytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSPK 21 antibody
    • HsPK21 antibody
    • NEK 2 antibody
    • NEK2 antibody
    • NEK2_HUMAN antibody
    • NEK2A antibody
    • Never in mitosis A-related kinase 2 antibody
    • NIMA (never in mitosis gene a) related kinase 2 antibody
    • NimA like protein kinase 1 antibody
    • NIMA related kinase 2 antibody
    • NimA related protein kinase 2 antibody
    • NimA-like protein kinase 1 antibody
    • NimA-related protein kinase 2 antibody
    • NLK 1 antibody
    • NLK1 antibody
    • Serine/threonine-protein kinase Nek2 antibody
    see all

Anti-NEK2 antibody images

  • ab115731 at 2.5ug/ml staining NEK2 in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115731 at 2.5ug/ml staining NEK2 in Human colon tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-NEK2 antibody (ab115731)

ab115731 has not yet been referenced specifically in any publications.

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