Overview

  • Product name
  • Description
    Sheep polyclonal to NEK2
  • Specificity
    This antibody recognises both splice variants of human NEK2 (NEK2A and NEK2B).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NLERRGRQLGEPEK

    , corresponding to amino acids 282-295 of Human NEK2

Properties

Applications

Our Abpromise guarantee covers the use of ab79903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • Function
    Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
  • Tissue specificity
    Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage
    Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
  • Post-translational
    modifications
    It is unsure whether Thr-170 or Ser-171 is phosphorylated.
  • Cellular localization
    Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSPK 21 antibody
    • HsPK21 antibody
    • NEK 2 antibody
    • NEK2 antibody
    • NEK2_HUMAN antibody
    • NEK2A antibody
    • Never in mitosis A-related kinase 2 antibody
    • NIMA (never in mitosis gene a) related kinase 2 antibody
    • NimA like protein kinase 1 antibody
    • NIMA related kinase 2 antibody
    • NimA related protein kinase 2 antibody
    • NimA-like protein kinase 1 antibody
    • NimA-related protein kinase 2 antibody
    • NLK 1 antibody
    • NLK1 antibody
    • Serine/threonine-protein kinase Nek2 antibody
    see all

Images



  • Predicted band size : 49 kDa
    Observed band size : 49 kDa

References

ab79903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab79903.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up