Anti-NEK2 antibody (ab79903)

Overview

• Product name
  Anti-NEK2 antibody
  See all NEK2 primary antibodies
• Description
  Sheep polyclonal to NEK2
• Specificity
  This antibody recognises both splice variants of human NEK2 (NEK2A and NEK2B).
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide:

  NLERRGRQLGEPEK

  , corresponding to amino acids 282-295 of Human NEK2

  Run BLAST with Run BLAST with

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.1% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Purity
  IgG fraction
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Other

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

  • Cancer
  • Cell cycle
  • Cell division

Applications

Target

• Function
  Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
• Tissue specificity
  Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
• Sequence similarities
  Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
  Contains 1 protein kinase domain.
• Developmental stage
  Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
• Post-translational
  modifications
  It is unsure whether Thr-170 or Ser-171 is phosphorylated.
• Cellular localization
  Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.

• Target information above from: UniProt accession P51955 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 4751 Human
  • Omim: 604043 Human
  • SwissProt: P51955 Human
  • Unigene: 153704 Human
• Alternative names
  • HSPK 21 antibody
  • HsPK21 antibody
  • NEK 2 antibody

  • NEK2 antibody
  • NEK2_HUMAN antibody
  • NEK2A antibody
  • Never in mitosis A-related kinase 2 antibody
  • NIMA (never in mitosis gene a) related kinase 2 antibody
  • NimA like protein kinase 1 antibody
  • NIMA related kinase 2 antibody
  • NimA related protein kinase 2 antibody
  • NimA-like protein kinase 1 antibody
  • NimA-related protein kinase 2 antibody
  • NLK 1 antibody
  • NLK1 antibody
  • Serine/threonine-protein kinase Nek2 antibody

  see all

Protocols

• Western blot protocols