Anti-NEK2 antibody [EPR3787] (ab109283)


  • Product name
    Anti-NEK2 antibody [EPR3787]
    See all NEK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3787] to NEK2
  • Host species
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NEK2

  • Positive control
    • Jurkat, 293T and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
    • Tissue specificity
      Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
    • Sequence similarities
      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Developmental stage
      Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
    • Post-translational
      It is unsure whether Thr-170 or Ser-171 is phosphorylated.
    • Cellular localization
      Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSPK 21 antibody
      • HsPK21 antibody
      • NEK 2 antibody
      • NEK2 antibody
      • NEK2_HUMAN antibody
      • NEK2A antibody
      • Never in mitosis A-related kinase 2 antibody
      • NIMA (never in mitosis gene a) related kinase 2 antibody
      • NimA like protein kinase 1 antibody
      • NIMA related kinase 2 antibody
      • NimA related protein kinase 2 antibody
      • NimA-like protein kinase 1 antibody
      • NimA-related protein kinase 2 antibody
      • NLK 1 antibody
      • NLK1 antibody
      • Serine/threonine-protein kinase Nek2 antibody
      see all


    • All lanes : Anti-NEK2 antibody [EPR3787] (ab109283) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa


    ab109283 has not yet been referenced specifically in any publications.

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