• Product nameAnti-NEK2 antibody [EPR3787]
    See all NEK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3787] to NEK2
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NEK2

  • Positive control
    • Jurkat, 293T and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionProtein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
    • Tissue specificityIsoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
    • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Developmental stageAccumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
    • Post-translational
      It is unsure whether Thr-170 or Ser-171 is phosphorylated.
    • Cellular localizationCytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSPK 21 antibody
      • HsPK21 antibody
      • NEK 2 antibody
      • NEK2 antibody
      • NEK2_HUMAN antibody
      • NEK2A antibody
      • Never in mitosis A-related kinase 2 antibody
      • NIMA (never in mitosis gene a) related kinase 2 antibody
      • NimA like protein kinase 1 antibody
      • NIMA related kinase 2 antibody
      • NimA related protein kinase 2 antibody
      • NimA-like protein kinase 1 antibody
      • NimA-related protein kinase 2 antibody
      • NLK 1 antibody
      • NLK1 antibody
      • Serine/threonine-protein kinase Nek2 antibody
      see all

    Anti-NEK2 antibody [EPR3787] images

    • All lanes : Anti-NEK2 antibody [EPR3787] (ab109283) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 52 kDa

    References for Anti-NEK2 antibody [EPR3787] (ab109283)

    ab109283 has not yet been referenced specifically in any publications.

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