Overview

  • Product name
  • Description
    Rabbit polyclonal to NEK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-255 of Human NEK3 (Uniprot ID: P51956).

  • Positive control
    • HepG2 whole cell lysate; Human Cal27 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer

Target

  • Function
    Kinase that may play a role in mitotic regulation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Form
    Cellular localisation: Cytoplasm; Cell projection › axon
  • Alternative names
    • HSPK 36 antibody
    • NEK3 antibody
    • NEK3_HUMAN antibody
    • Never in mitosis A-related kinase 3 antibody
    • NIMA (never in mitosis gene a) related kinase 3 antibody
    • NimA related protein kinase 3 antibody
    • NimA-related protein kinase 3 antibody
    • Serine/threonine protein kinase Nek3 antibody
    • Serine/threonine-protein kinase Nek3 antibody
    see all

Images

  • Anti-NEK3 antibody (ab154536) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling NEK3 with ab154536 at 1/500 dilution.

References

ab154536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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