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Synthetic peptide designed within residues: FTQMCLGVNH IHKKRVLHRD IKSKNIFLTQ NGKVKLGDFG SARLLSNPMA, corresponding to N terminal amino acids 108-157 of Human NEK3 (NP_002489)
Our Abpromise guarantee covers the use of ab83221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
ab83221 has not yet been referenced specifically in any publications.