Overview

  • Product nameAnti-NEK4 antibody
    See all NEK4 primary antibodies
  • Description
    Rabbit polyclonal to NEK4
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 475-525 of human NEK4 (NP_003148.2)

  • Positive control
    • HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95176 was affinity purified using an epitope specific to NEK4 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceNEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • MGC33171 antibody
    • Never in mitosis A related kinase 4 antibody
    • Never in mitosis gene A related kinase 4 antibody
    • NIMA (never in mitosis gene a) related kinase 4 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
    • NIMA related kinase 4 antibody
    • NimA related protein kinase 4 antibody
    • NLK2 antibody
    • NRK2 antibody
    • OTTHUMP00000213582 antibody
    • Pp12301 antibody
    • Serine/threonine kinase 2 antibody
    • Serine/threonine protein kinase 2 antibody
    • Serine/threonine protein kinase Nek4 antibody
    • Serine/threonine protein kinase NRK2 antibody
    • STK2 antibody
    see all

Anti-NEK4 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg/ml
    Lane 2 : HeLa cell lysate at 15 µg/ml
    Lane 3 : HeLa cell lysate at 5 µg/ml


    Predicted band size : 95 kDa
    Detection: Chemiluminescence with exposure times of 3 minutes.
  • Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgG
    Detection: Chemiluminescence with exposure times of 30 seconds.

References for Anti-NEK4 antibody (ab95176)

ab95176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"