Purification notesab95176 was affinity purified using an epitope specific to NEK4 immobilized on solid support.
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceNEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
Western blot - NEK4 antibody (ab95176)
All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg/ml Lane 2 : HeLa cell lysate at 15 µg/ml Lane 3 : HeLa cell lysate at 5 µg/ml
Predicted band size : 95 kDa Detection: Chemiluminescence with exposure times of 3 minutes.
Immunoprecipitation - NEK4 antibody (ab95176)
Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgG Detection:
Chemiluminescence with exposure times of 30 seconds.
References for Anti-NEK4 antibody (ab95176)
has not yet been referenced specifically in any publications.
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