Overview

  • Product nameAnti-NEK4 antibody
    See all NEK4 primary antibodies
  • Description
    Rabbit polyclonal to NEK4
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 350-400 (KQDLSNTTEL ATISSVNIDI LPAKGRDSVS DGFVQENQPR YLDASNELGG I) of Human NEK4 (NP_003148.2).

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95189 was affinity purified using an epitope specific to NEK4 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab95189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceNEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • EC 2.7.11.1 antibody
      • MGC33171 antibody
      • Never in mitosis A related kinase 4 antibody
      • Never in mitosis gene A related kinase 4 antibody
      • NIMA (never in mitosis gene a) related kinase 4 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
      • NIMA related kinase 4 antibody
      • NimA related protein kinase 4 antibody
      • NLK2 antibody
      • NRK2 antibody
      • OTTHUMP00000213582 antibody
      • Pp12301 antibody
      • Serine/threonine kinase 2 antibody
      • Serine/threonine protein kinase 2 antibody
      • Serine/threonine protein kinase Nek4 antibody
      • Serine/threonine protein kinase NRK2 antibody
      • STK2 antibody
      see all

    Anti-NEK4 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95189 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of NEK4 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95189 at 3 µg/mg lysate for IP. An anti-NEK4 antibody, which recognizes a downstream epitope, was used at 1µg/ml for the subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-NEK4 antibody (ab95189)

    ab95189 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"