The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
RelevanceNEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95189 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - NEK4 antibody (ab95189)
Detection of NEK4 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95189 at 3 µg/mg lysate for IP. An anti-NEK4 antibody, which recognizes a downstream epitope, was used at 1µg/ml for the subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-NEK4 antibody (ab95189)
has not yet been referenced specifically in any publications.
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