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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 95 kDa.
1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
Relevance NEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
Cellular localization Nuclear
EC 188.8.131.52 antibody
Never in mitosis A related kinase 4 antibody
Anti-NEK4 antibody images
Western blot - NEK4 antibody (ab96607)
Anti-NEK4 antibody (ab96607) at 1/1000 dilution + A431 whole cell lysate at 30 µg Predicted band size : 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NEK4 antibody (ab96607)
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab96607 at 1:100 dilution.
References for Anti-NEK4 antibody (ab96607)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"