Overview

  • Product nameAnti-NEK5 antibody
    See all NEK5 primary antibodies
  • Description
    Mouse monoclonal to NEK5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 601-708 of Human NEK5 with a proprietary tag.

  • Positive control
    • IMR-32 cell lysate; Recombinant tagged human NEK5 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC75495 antibody
    • NEK5 antibody
    • NEK5_HUMAN antibody
    • Never in mitosis A-related kinase 5 antibody
    • NIMA (never in mitosis gene a) related kinase 5 antibody
    • NIMA related kinase 5 antibody
    • NimA related protein kinase 5 antibody
    • NimA-related protein kinase 5 antibody
    • OTTHUMP00000040896 antibody
    • Serine/threonine protein kinase Nek5 antibody
    • Serine/threonine-protein kinase Nek5 antibody
    see all

Anti-NEK5 antibody images

  • Anti-NEK5 antibody (ab77451) at 1 µg/ml + Recombinant tagged human NEK5 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 81 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-NEK5 antibody (ab77451) at 1 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NEK5 antibody (ab77451)

ab77451 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77451.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"