• Product nameAnti-NEK6 antibody
    See all NEK6 primary antibodies
  • Description
    Rabbit polyclonal to NEK6
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human NEK6

  • Positive control
    • Fetal lung lysate



Our Abpromise guarantee covers the use of ab76071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.


  • FunctionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
  • Tissue specificityUbiquitous, with highest expression in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
    • NEK6 antibody
    • NEK6_HUMAN antibody
    • Never in mitosis A-related kinase 6 antibody
    • NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody
    • NimA related protein kinase 6 antibody
    • NimA-related protein kinase 6 antibody
    • Protein kinase SID6 1512 antibody
    • Protein kinase SID6-1512 antibody
    • Putative serine threonine protein kinase antibody
    • Serine/threonine protein kinase Nek6 antibody
    • Serine/threonine-protein kinase Nek6 antibody
    • SID6 1512 antibody
    • SID61512 antibody
    see all

Anti-NEK6 antibody images

  • Anti-NEK6 antibody (ab76071) at 1 µg/ml + fetal lung lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • IHC image of ab76071 staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76071, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NEK6 antibody (ab76071)

This product has been referenced in:
  • Bian Z  et al. Never in mitosis gene A related kinase-6 attenuates pressure overload-induced activation of the protein kinase B pathway and cardiac hypertrophy. PLoS One 9:e96095 (2014). Mouse, Human . Read more (PubMed: 24763737) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 airway epithelial cell line)
Loading amount 25 µg
Specification A549 airway epithelial cell line
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Feb 09 2012