Recombinant
RabMAb

Anti-NEK6 antibody [EPR5282] (ab109177)

Overview

  • Product name
    Anti-NEK6 antibody [EPR5282]
    See all NEK6 primary antibodies
  • Description
    Rabbit monoclonal [EPR5282] to NEK6
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NEK6

  • Positive control
    • HepG2, HeLa, 293T and HT-1080 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
    • Tissue specificity
      Ubiquitous, with highest expression in heart and skeletal muscle.
    • Sequence similarities
      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
      • NEK6 antibody
      • NEK6_HUMAN antibody
      • Never in mitosis A-related kinase 6 antibody
      • NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody
      • NimA related protein kinase 6 antibody
      • NimA-related protein kinase 6 antibody
      • Protein kinase SID6 1512 antibody
      • Protein kinase SID6-1512 antibody
      • Putative serine threonine protein kinase antibody
      • Serine/threonine protein kinase Nek6 antibody
      • Serine/threonine-protein kinase Nek6 antibody
      • SID6 1512 antibody
      • SID61512 antibody
      see all

    Images

    • All lanes : Anti-NEK6 antibody [EPR5282] (ab109177) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 36 kDa

    References

    ab109177 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109177.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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