Overview

  • Product name
  • Description
    Mouse polyclonal to NEK7
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-302 of Human NEK7

  • Positive control
    • NEK7 transfected mammalian cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).

Target

  • Tissue specificity
    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • NEK7 antibody
    • NEK7_HUMAN antibody
    • Never in mitosis A-related kinase 7 antibody
    • Never in mitosis gene a-related kinase 7 antibody
    • NIMA (never in mitosis gene a) related kinase 7 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
    • NimA related protein kinase 7 antibody
    • NIMA-related kinase 7 antibody
    • NimA-related protein kinase 7 antibody
    • Serine/threonine-protein kinase Nek7 antibody
    see all

Images

  • All lanes : Anti-NEK7 antibody (ab68060) at 1/500 dilution

    Lane 1 : NEK7 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 34 kDa
    Observed band size: 28 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). Read more (PubMed: 25093993) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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