Anti-NEK7 antibody (ab80948)


  • Product nameAnti-NEK7 antibody
    See all NEK7 primary antibodies
  • Description
    Rabbit polyclonal to NEK7
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (DAKARADCIK EIDLLKQLNH PNVIKYYASF IEDNELNIVL ELADAGDLSR) of Human NEK7 (NP_598001).

  • Positive control
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab80948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.


Anti-NEK7 antibody images

  • Anti-NEK7 antibody (ab80948) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • All lanes : Anti-NEK7 antibody (ab80948) at 1/2000 dilution

    Lane 1 : Mouse brain tissue cytoplasmic lysate
    Lane 2 : Mouse brain tissue cytoplasmic lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35,38 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Dr Maryna Polyakova

    Blocked with 5% non-fat Milk for 1 hour at 18°C


    Secondary antibody - anti-rabbit HRP (ab6721)

    See Abreview

References for Anti-NEK7 antibody (ab80948)

ab80948 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain Coronal sections)
Specification Brain Coronal sections

Abcam user community

Verified customer

Submitted Apr 15 2016

Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Dr. Maryna Polyakova

Verified customer

Submitted May 15 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

Read More

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application o...

Read More