Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - NEK7 antibody (ab95873)
All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Predicted band size : 35 kDa
Immunoprecipitation - NEK7 antibody (ab95873)
Detection of NEK7 by Western Blot of Immunprecipitate.
(1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds.