Overview

  • Product name
  • Description
    Rabbit polyclonal to NEK7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 238 and 302 of Human NEK7.

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Tissue specificity
    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
  • Sequence similarities
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • NEK7 antibody
    • NEK7_HUMAN antibody
    • Never in mitosis A-related kinase 7 antibody
    • Never in mitosis gene a-related kinase 7 antibody
    • NIMA (never in mitosis gene a) related kinase 7 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
    • NimA related protein kinase 7 antibody
    • NIMA-related kinase 7 antibody
    • NimA-related protein kinase 7 antibody
    • Serine/threonine-protein kinase Nek7 antibody
    see all

Images

  • Anti-NEK7 antibody (ab96538) at 1/500 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 35 kDa

  • Top panel: ab96538, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by immunofluorescence.
    Bottom panel: merged with DNA probe.

References

This product has been referenced in:
  • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). Read more (PubMed: 25093993) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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