Validated using a knockout cell line
Recombinant
RabMAb

Anti-NEK7 antibody [EPR4901] (ab109433)

Overview

  • Product name
    Anti-NEK7 antibody [EPR4901]
    See all NEK7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4901] to NEK7
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Human brain, HeLa, A549, HepG2, fetal liver, and Jurkat lysates; Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
Flow Cyt 1/100 - 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Tissue specificity
      Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
    • Sequence similarities
      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.11.1 antibody
      • NEK7 antibody
      • NEK7_HUMAN antibody
      • Never in mitosis A-related kinase 7 antibody
      • Never in mitosis gene a-related kinase 7 antibody
      • NIMA (never in mitosis gene a) related kinase 7 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
      • NimA related protein kinase 7 antibody
      • NIMA-related kinase 7 antibody
      • NimA-related protein kinase 7 antibody
      • Serine/threonine-protein kinase Nek7 antibody
      see all

    Images



    • Predicted band size : 35 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
      Lane 3: A549 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Fetal liver lysate
      Lane 6 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
    • Flow cytometric analysis of NEK7 in permeabilized Jurkat cells using ab109433 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab109433 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109433.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up