Overview

  • Product nameAnti-NEK9 antibody
    See all NEK9 primary antibodies
  • Description
    Mouse monoclonal to NEK9
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 226-326 of Human NEK9

Properties

Applications

Our Abpromise guarantee covers the use of ab57925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
    • Tissue specificityMost abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
    • Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
      Contains 6 RCC1 repeats.
    • Developmental stageExpression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.
    • DomainDimerizes through its coiled-coil domain.
    • Post-translational
      modifications
      Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1995 antibody
      • APUG antibody
      • DKFZp434D0935 antibody
      • LCCS10 antibody
      • MGC138306 antibody
      • MGC16714 antibody
      • NC antibody
      • NEK 9 antibody
      • Nek8 antibody
      • nek9 antibody
      • NEK9_HUMAN antibody
      • Nercc 1 kinase antibody
      • NERCC antibody
      • NERCC1 antibody
      • Nercc1 kinase antibody
      • Never in mitosis A related kinase 9 antibody
      • Never in mitosis A-related kinase 9 antibody
      • NIMA (never in mitosis gene a) related kinase 9 antibody
      • NIMA related kinase 8 antibody
      • NIMA related kinase 9 antibody
      • NIMA related kinase Nek8 antibody
      • NimA related protein kinase 9 antibody
      • NIMA-related kinase 8 antibody
      • NimA-related protein kinase 9 antibody
      • Serine/threonine protein kinase Nek9 antibody
      • Serine/threonine-protein kinase Nek9 antibody
      see all

    Anti-NEK9 antibody images



    • Predicted band size : 107 kDa
      NEK9 antibody (ab57925) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-NEK9 antibody (ab57925)

    This product has been referenced in:
    • Cooper MJ  et al. Application of multiplexed kinase inhibitor beads to study kinome adaptations in drug-resistant leukemia. PLoS One 8:e66755 (2013). WB . Read more (PubMed: 23826126) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"